Author/Editor		Ranković, Branislava; Zidar, Nina; Žlajpah, Margareta; Boštjančič, Emanuela
Title		Epithelial-mesenchymal transition-related micrornas and their target genes in colorectal cancerogenesis
Type		članek
Vol. and No.		Letnik 8, št. 10
Publication year		2019
Volume		str. 1-22
ISSN		2077-0383 - Journal of clinical medicine
Language		eng
Abstract		MicroRNAs of the miR-200 family have been shown experimentally to regulate epithelialmesenchymal transition (EMT). Although EMT is the postulated mechanism of development and progression of colorectal cancer (CRC), there are still limited and controversial data on expression of miR-200 family and their target genes during CRC cancerogenesis. Our study included formalinfixed paraffin-embedded biopsy samples of 40 patients (10 adenomas and 30 cases of CRC with corresponding normal mucosa). Expression of miR-141, miR-200a/b/c and miR-429 and their target genes (CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2 and ZEB2) was analysed using quantitative real-time PCR. Expression of E-cadherin was analysed using immunohistochemistry. All miRNAs were down-regulated and their target genes showed the opposite expression in CRC compared to adenoma. Down-regulation of the miR-200 family at the invasive front in comparison to the central part of tumour was observed as well as a correlation of expression of miR-200b, CDKN1B, ONECUT2 and ZEB2 expression to nodal metastases. Expression of the miR-200 family and SOX2 also correlated with E-cadherin staining. These results suggest that the miR-200 family and their target genes contribute to progression of adenoma to CRC, invasive properties and development of metastases. Our results strongly support the postulated hypotheses of partial EMT and intra-tumour heterogeneity during CRC cancerogenesis.
Keywords		colorectal adenoma colorectal carcinoma metastases colorectal adenoma kolorektalni karcinom metastaze