Author/Editor		Goličnik, Marko; Bavec, Aljoša
Title		Evaluation of the paraoxonase-1 kinetic parameters of the lactonase activity by nonlinear fit of progress curves
Type		članek
Vol. and No.		Letnik 35, št. 1
Publication year		2020
Volume		str. str. 261-264
ISSN		1475-6374 - Journal of enzyme inhibition and medicinal chemistry
Language		eng
Abstract		Although paraoxonase-1 (PON1) activity has been demonstrated to be a reliable biomarker of various diseases, clinical studies have been based only on relative comparison of specific enzyme activities, which capture differences mainly due to (usually unknown) PON1 concentration. Hence, the aim of this report is to present for the first time the simple evaluation method for determining autonomous kinetic parameter of PON1 that could be also associated with polymorphic forms and diseases; i.e. the Michaelis constant which is enzyme concentration independent quantity. This alternative approach significantly reduces the number of experiments needed, and it yields the results with great accuracy.
Keywords		lactonase activity paraoxonase-1 evaluation of kinetic parameters laktonazna aktivnost paraoksonaza-1 vrednotenje kinetičnih parametrov