Author/Editor		Mason, WT; Dempster, J; Zorec, R; Hoyland, J; Lledo, PM
Title		Fluorescence measurements of cytosolic calcium: combined photometry with electrophysiology
Type		članek
Source		Methods Neurosci
Vol. and No.		Letnik 27
Publication year		1995
Volume		str. 81-122
Language		eng
Abstract		The development of optical probes for biological activity combined with powerful photon aquisition and analysis technology is having a major effect on the study of electrophysiology parameters of single living cells. The best of these probes appear not to interfere with normal cellular processes, although they do rquire caution in interpretation of data, because the probes may localize within cells, rendering them insensitive or modifying their properties.In thes case of ions, photometric technology allows the application of these probes to yield data that will shed light on the relationship between ion entry and ionic concentration inside the cell on very fast time scales. New real-time imaging probes for ions, cyclic mucleotide, cellular enzymes and genetic material are under development and appear to be suitable for work on living cells, all of which will further extend our capability to study cellular homeostasis and its perturbation in health and disease states. A variety of commercial systems are available, making entry into this area of research both feasible and cost-effective.
Descriptors		CALCIUM FLUORESCENT DYES CYTOSOL ELECTROPHYSIOLOGY PHOTOMETRY ION TRANSPORT