Author/Editor		Kastelic-Suhadolc, T; Lenasi, H
Title		Androgen binding proteins in the plasma membrane of Cochliobolus lunatus
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 42, št. 2
Publication year		1995
Volume		str. 199-206
Language		eng
Abstract		Androgen binding proteins were found in the plasma membrane prepared from protoplasts of the filamentous fungus Cochliobolus lunatus. The binding was studied by competitive binding assay using (3H) labeled and nonlabeled androgens. The mean Kd determined for testosterone binding protein (TBP) by Scatchard analysis was 11.1. nM and the binding capacity Bmax ranged from 34 to 56 fmol/mg protein; for 4-androstene-3,17-dione (androstenedione) binding protein (ABP) the Kd was 7.4 nM and Bmax ranged from 20 to 59 fmol/mg protein. The steroid binding specificity for TBP and ABP was studied with different competitors. Data for binding specificity of fungal and mammalian androgen binding proteins were compared and a characteristic low specificity for androgen binding proteins of C. lunatus was observed.
Descriptors		ASCOMYCETES ANDROGEN-BINDING PROTEINS SEX HORMONE-BINDING GLOBULIN SENSITIVITY AND SPECIFICITY BINDING, COMPETITIVE CELL MEMBRANE