Author/Editor		Jevšnik, Monika; Lusa, Lara; Uršič, Tina; Glinšek Biškup, Urška; Petrovec, Miroslav
Title		Detection of herpes simplex and varicella-zoster virus from skin lesions
Type		članek
Publication year		2020
Volume		str. str.
ISSN		0732-8893 - Diagnostic Microbiology and Infectious Disease
Language		eng
Abstract		We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicellazoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana% instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa=1). Concordance between Solana HSV1+2/VZV and RT-PCR was 98.3% (kappa=0.95) for HSV-1 and 99.3% (kappa=0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
Keywords		herpes simplex virus varicella-zoster virus skin lesion herpes simplex virus virus varicella-zoster lezija kože