Author/Editor		Marc-Malovrh, Mateja; Camlek, Luka; Škrgat, Sabina; Kern, Izidor; Fležar, Matjaž; Dežman, Manca; Korošec, Peter
Title		Elevated eosinophils, IL5 and IL8 in induced sputum in asthma patients with accelerated FEV1 decline
Type		članek
Vol. and No.		, št. Vol. 162
Publication year		2020
Volume		str. [1-8]
ISSN		1532-3064 - Respiratory medicine
Language		eng
Abstract		Background: Some patients with asthma present with accelerated lung function decline. This phenomenon is mostly associated with severe exacerbations and with poor asthma control. Objective: Our aim was to detect the extent of FEV1 decline in patients with mild asthma and to discriminate clinical, functional and inflammatory factors associated with accelerated FEV1 decline. Methods: We recruited 50 patients with mild asthma for pulmonary function testing and induced sputum sampling 12-15 years after the initial diagnosis. In 33 patients, from whom sputum of a good quality was obtained, inflammatory cells were counted and concentrations of cytokines IL-2, IL-4, IL-5, IL-8, IL-10, IFN-[gamma], angiogenin and VEGF in the sputum were measured by cytometric bead array. Results: Eighteen of 33 patients presented with accelerated FEV1 decline of more than 30 ml/year, with a mean (SEM) of 43.2 (3.9) ml/year, compared to 15 control patients with a FEV1 decline of 14.4 (2.1) ml/year. In the accelerated FEV1 decline group, we found elevated sputum levels of IL5 with a median (IQR) of 1.8 (0.4-3.2) pg/ ml vs. 0.2 (0.1-1.2) pg/ml, p = 0.04; IL8 with a mean (SEM) of 1503 (194) pg/ml vs. 938 (177) pg/ml, p = 0.04; and eosinophils with a median (IQR) of 223 (41-1020) cells/[micro]l vs. 39 (1-190) cells/[micro]l, p = 0.03. No significant differences in other measured parameters were detected between the two groups. Conclusion: Elevated sputum eosinophils, IL5 and IL8, which have a potential to stimulate airway remodelling, might be a useful non-invasive biomarkers and therapeutic targets of accelerated FEV1 decline in asthma patients.
Descriptors		Asthma Respiratory function tests Eosinophils Interleukin-5 Interleukin-8 Sputum Astma Testi respiratorne funkcije Eozinofilci Interlevkin-5 Interlevkin-8 Izmeček Diagnosis Diagnostika
Keywords		upad pljučne funkcije FEV1 induced sputum asthma control lung function decline FEV1 inducirani izmeček nadzor astme