Author/Editor		Lisjak, Marjeta; Potokar, Maja; Zorec, Robert; Jorgačevski, Jernej
Title		Indirect role of AQP4b and AQP4d isoforms in dynamics of astrocyte volume and orthogonal arrays of particles
Type		članek
Vol. and No.		Letnik 9, št. 3
Publication year		2020
Volume		str. 1-17
ISSN		2073-4409 - Cells (Basel, Switzerland)
Language		eng
Abstract		Water channel aquaporin 4 (AQP4) plays a key role in the regulation of water homeostasis in the central nervous system (CNS). It is predominantly expressed in astrocytes lining blood-brain and blood-liquor boundaries. AQP4a (M1), AQP4c (M23), and AQP4e, present in the plasma membrane, participate in the cell volume regulation of astrocytes. The function of their splicing variants, AQP4b and AQP4d, predicted to be present in the cytoplasm, is unknown.We examined the cellular distribution of AQP4b and AQP4d in primary rat astrocytes and their role in cell volume regulation. The AQP4b and AQP4d isoforms exhibited extensive cytoplasmic localization in early and late endosomes/lysosomes and in the Golgi apparatus. Neither isoform localized to orthogonal arrays of particles (OAPs) in the plasma membrane. The overexpression of AQP4b and AQP4d isoforms in isoosmotic conditions reduced the density of OAPs; in hypoosmotic conditions, they remained absent from OAPs. In hypoosmotic conditions, the AQP4d isoform was significantly redistributed to early endosomes, which correlated with the increased trafficking of AQP4-laden vesicles. The overexpression of AQP4d facilitated the kinetics of cell swelling, without affecting the regulatory volume decrease. Therefore, although they reside in the cytoplasm, AQP4b and AQP4d isoforms may play an indirect role in astrocyte volume changes.
Keywords		aquaporin endosome astrocyte volume akvaporin endosom prostornina astrocitov