Author/Editor		Khurana, Preeti; Kolundzic, Nikola; Rogar, Marija; Hobbs, Carl; Wong, X. F. Colin C.; Common, John E. A.; Ilic, Dusko; Liović, Mirjana
Title		Stem cell research lab resource
Type		članek
Vol. and No.		, št. Vol. 45
Publication year		2020
Volume		str. 1-5
ISSN		1876-7753 - Stem cell research
Language		eng
Abstract		We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle kera-tinocytes of a healthy male characterized with a maximum number offilaggrin tandem repeats, making this iPSCline the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consistedof molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics tech-nology and next-generation sequencing of thefilaggrin alleles, and pluripotency and differentiation potentialstesting by immunofluorescence of associated markers bothin vitroandin vivo. The MLi-003A line has been alsotested for ability to differentiate into keratinocytes.
Keywords		pluripotencija keratinociti atopijski dermatitis pluripotency keratinocyte atopic dermatitis