Author/Editor		Podgoršek, Daša; Ružić-Sabljić, Eva; Logar, Mateja; Pavlović, Andrea; Remec-Zafred, Tatjana; Baklan, Zvonko; Cerar Kišek, Tjaša
Title		Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection
Type		članek
Vol. and No.		Letnik 20, št. 1
Publication year		2020
Volume		str. 1-9
ISSN		1471-2180 - BMC microbiology
Language		eng
Abstract		Background: Different diagnostic methods have been used for the laboratory confirmation of leptospirosis. Molecular diagnostic techniques are not only faster and more sensitive than culture analysis, but can also detect a Leptospira infection before the appearance of antibodies. The aim of the present study was to analyze and compare two different PCR approaches applied to blood and urine specimens obtained from patients with clinical manifestations that were suggestive of leptospirosis. Furthermore, the results of these different PCR approaches were compared with the results of culture and serology analyses. Results: A total of 400 samples (234 blood or 58.5% and 166 urine of 41.5%) from 310 Slovenian patients with clinical manifestations suggestive of leptospirosis were tested using conventional PCR assays targeting the rrs gene and RT-PCR targeting the lipL32 gene. Additionally, culture, serology and sequence analysis were performed for the majority of these samples. The PCR and RT-PCR results were concordant in 376 out of 400 of these samples (94.0%). Conventional PCR was positive for 27 out of 400 samples (6.8%) and RT-PCR was positive for 47 out of 400 samples (11.8%). Culture and microscopic agglutination tests supported these diagnoses. Conclusions: A comparison of the two PCR methods indicated that the RT-PCR targeting of the lipL32 gene was faster, more sensitive and more specific for the determination of Leptospira DNA in these clinical samples.
Keywords		leptospira leptospirosis Lipl32 leptospira leptospiroza Lipl32