Author/Editor		Justin, Mojca; Jež, Mojca; Košir, Andrej; Miceska, Simona; Rožman, Primož; Jazbec, Katerina
Title		Application of the 3R principles
Type		članek
Publication year		2020
Volume		str. str.
ISSN		1758-1117 - Laboratory animals
Language		eng
Abstract		Experimental murine models are an essential tool in the field of bone marrow (BM) transplantation research.Therefore, numerous mice are required to obtain a sufficient number of BM cells, which is in contrast withthe Reduction principle of the 3R principles. The selection of the cell source and the isolation protocol aretherefore critical in obtaining a sufficient yield of cells for experiments. Nowadays, the vertebrae are alreadyused as an extra source of BM cells to enrich the number of isolated cells from the long bones and ilia (LBI),when needed. Yet, little is known if BM cells from LBI and vertebrae share the same characteristics and canbe pooled together for further analysis. Therefore, in this study, we aimed to compare the quantity andcharacteristics of haematopoietic and stromal cell lines in the BM from the LBI and vertebrae. To counthaematopoietic and mesenchymal stem/stromal progenitors, colony-forming unit assays were performed.To determine the expansion capacity of mesenchymal stem/stromal cells (MSCs), cultivation of MSCs andmeasurement of the expression of surface markers by flow cytometry was performed. The characterisationand enumeration of immune cell populations was also performed by flow cytometry. Here, we show that thevertebrae are a comparable source of BM cells to the LBI regarding the analysed parameters.
Keywords		animal research bone marrow bone crush raziskave na živalih kostni mozeg drobljenje kosti