Author/Editor		Fink, Katja; Lobe Prebil, Mateja; Vardjan, Nina; Jensen, J; Zorec, Robert; Kreft, Marko
Title		Increase in subcellular GSK-3 clusters in insulin- and adrenaline-treated differenti ated rat skeletal muscle fibres
Type		članek
Vol. and No.		Letnik 39, št. 1
Publication year		2020
Volume		str. 25-32
ISSN		1580-3139 - Image Analysis & Stereology online journal (Printed version)
Language		eng
Abstract		Glycogen synthase kinase 3 (GSK-3) plays an important role in m etabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation . The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisatio n in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully diffe rentiated muscle fibre by immunofluores- cence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of si ngle central optical section of t he muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average siz e were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linea rly related to their quantity. We conclude that subcellular GSK-3 in isolate d skeletal muscle fibres is lo calized in clusters and clustering increased after stimulation with insulin and/or adrenaline.
Keywords		adrenaline glycogen synthase kinase 3 skeletal muscle fibre adrenalin glikogen sintaza kinaza 3 skeletna mišična vlakna