Author/Editor		Skubic, Cene; Vovk, Irena; Rozman, Damjana; Križman, Mitja
Title		Simplified LC-MS method for analysis of sterols in biological samples
Type		članek
Vol. and No.		Letnik 25, št. 18
Publication year		2020
Volume		str. 1-10
ISSN		1420-3049 - Molecules (Basel, Switzerland)
Language		eng
Abstract		We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes in a single chromatographic run using a pentafluorophenyl (PFP) stationary phase. The method also avails on a minimized sample preparation procedure in order to obtain a relatively high sample throughput. The method was validated on 10 sterol standards that were detected in a single chromatographic LC-MS run without derivatization. Our developed method can be used in research or clinical applications for disease-related detection of accumulated cholesterol intermediates. Disorders in the late part of cholesterol synthesis lead to severe malformation in human patients. The developed method enables a simple, sensitive, and fast quantification of sterols, without the need of extended knowledge of the LC-MS technique, and represents a new analytical tool in the rising field of cholesterolomics.
Keywords		holesterol sinteza holesterola sterolni intermediati cholesterol cholesterol synthesis sterol intermediates