Author/Editor		Žlajpah, Margareta; Boštjančič, Emanuela; Zidar, Nina
Title		(Epi)genetic regulation of osteopontin in colorectal cancerogenesis
Type		članek
Vol. and No.		Letnik 12, št. 16
Publication year		2020
Volume		str. 1389-1403
ISSN		1750-1911 - Epigenomics
Language		eng
Abstract		Aim: To identify (epi)genetic regulators of osteopontin (OPN, encoded by SPP1 gene) from normal colon mucosa to adenoma, adenoma with early carcinoma and advanced carcinoma. Patients & methods: Biopsy samples of 41 patients with different patohistologic diagnosis were used. Using qPCR, pyrosequencing and statistical analysis, we determined the expression level of osteopontin regulatory miRNAs, its copy number and methylation status. Results & conclusion: We showed that hsa-miR-146a-5p expression is inversely proportional to the expression level of SPP1 and that expression might be also controlled by copy number and methylation. These results suggest that not only expression of SPP1 but also its copy number, methylation status and expression of its regulators might be used as a potential biomarker of colorectal cancer.
Keywords		kolorektalni adenom kolorektalni karcinom epigenetika colorectal adenoma colorectal carcinoma epigenetics