Author/Editor		Nieuwenhuijse, David F.; Munnink, Bas B. Oude; Phan, My V. T.; Munk, Patrick; Venkatakrishnan, Shweta; Aarestrup, Frank M.; Cotten, Matthew; Koopmans, Marion P. G.; Trkov, Marija
Title		Setting a baseline for global urban virome surveillance in sewage
Type		članek
Vol. and No.		Letnik 10, št. 1
Publication year		2020
Volume		str. 1-13
ISSN		2045-2322 - Scientific reports
Language		eng
Abstract		The rapid development of megacities, and their growing connectedness across the world is becoming a distinct driver for emerging disease outbreaks. Early detection of unusual disease emergence and spread should therefore include such cities as part of risk-based surveillance. A catch-all metagenomic sequencing approach of urban sewage could potentially provide an unbiased insight into the dynamics of viral pathogens circulating in a community irrespective of access to care, a potential which already has been proven for the surveillance of poliovirus. Here, we present a detailed characterization of sewage viromes from a snapshot of 81 high density urban areas across the globe, including in-depth assessment of potential biases, as a proof of concept for catch-all viral pathogen surveillance. We show the ability to detect a wide range of viruses and geographical and seasonal differences for specific viral groups. Our findings offer a cross-sectional baseline for further research in viral surveillance from urban sewage samples and place previous studies in a global perspective.
Keywords		odplake urbana območja globalni urbani nadzor sewage urban areas global urban surveillance