Author/Editor		Uhan, Sara; Zidar, Nina; Tomažič, Aleš; Hauptman, Nina
Title		Hypermethylated promoters of genes UNC5D and KCNA1 as potential novel diagnostic biomarkers in colorectal cancer
Type		članek
Publication year		2020
Volume		str. str.
ISSN		1750-1911 - Epigenomics
Language		eng
Abstract		Aim: Identification of aberrant hypermethylation in promoter regions of candidate genes to discover potential biomarkers for colorectal cancer. Materials & Methods: Genes BMP2, IRF4, KCNA1, LRRC7, NRG3, SLC27A6 and UNC5D were pre-selected in a bioinformatics study for their hypermethylation status in colorectal cancer. Methylation analysis was performed on 202 cancer tissue specimens to validate candidate genes. Results: Genes KCNA1 and UNC5D displayed methylation in 95.3 and 99.7% of The Cancer Genome Atlas dataset samples and in 96 and 98% of our experimentally tested samples, respectively. Conclusion: KCNA1 and UNC5D promoter hypermethylation holds diagnostic biomarker potential in patients with early colorectal cancer.
Keywords		kolorektalni karcinom metilacija DNA analiza taljenja z visoko ločljivostjo colorectal carcinoma DNA methylation high-resolution melting analysis