Author/Editor		Welsh, Joshua A.; Pol, Edwin; Bettin, Britta A.; Carter, David R. F.; Hendrix, An; Lenassi, Metka; Langlois, Marc-André; Llorente, Alicia; Nes, Arthur S.; Nieuwland, Rienk
Title		Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration
Type		članek
Vol. and No.		, št. Vol. 9
Publication year		2020
Volume		str. 1-18
ISSN		2001-3078 - Journal of extracellular vesicles
Language		eng
Abstract		Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV refractive index, epitope abundance, size and concentration. Due to their predominant use among EV researchers, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.
Keywords		extracellular vesicles exosomes microvesicles zunajcelični vezikli eksosomi mikrovezili