Author/Editor		Trbojević-Čepe, M
Title		Detection of IgG oligoclonal bands in unconcentrated CSF by isoelectric focusing in ultrathin-layer polyacrylamide gel, direct antiserum immunofixation and silver staining
Type		članek
Source		In: Vodušek DB, editor. Proceedings of the Symposium on multiple sclerosis with the 11th dr. Janez Faganel memorial lecture; 1995 Oct 6-7; Ljubljana. Ljubljana: University institute of clinical neurophysiology,
Publication year		1995
Volume		str. 25-33
Language		eng
Abstract		Isoelectric focusing of proteins (IEF) in ultrathin-layer polyacrylamide gel (0.4 mm, PAG), followed by direct immunofixation on gel with specific antiserum and silver staining, is a highly specific, sensitive and simple method for the demonstration of oligoclonal IgG bands in unconcentrated cerebrospinal fluid (CSF) samples (5-20 microI). For the present method, the optimal concentrations of IgG in CSF samples are about 0.015-0.020 g/I, corresponding to applied amount of 150-200 ng. The method appears to be a useful alternative for the demonstration of oligoclonal IgG bands in unconcentrated CSF samples, and can be recommended for use in CSF laboratory routine.
Descriptors		MULTIPLE SCLEROSIS IGG ISOELECTRIC FOCUSING SILVER STAINING