| Author/Editor | Vukasović, Andreja; Asnaghi, Maria Adelaide; Kostešić, Petar; Quasnichka, Helen; Cozzolino, Carmine; Pušić, Maja; Hails, Lauren; Trainor, Nuala; Krause, Christian; Figallo, Elisa | |
| Title | Bioreactor-manufactured cartilage grafts repair acute and chronic osteochondral defects in large animal studies | |
| Type | članek | |
| Vol. and No. | Letnik 52, št. 6 | |
| Publication year | 2019 | |
| Volume | str. 1-14 | |
| ISSN | 1365-2184 - Cell proliferation | |
| Language | eng | |
| Abstract | Objectives: Bioreactor-based production systems have the potential to overcome limitations associated with conventional tissue engineering manufacturing methods, facilitating regulatory compliant and cost-effective production of engineered grafts for widespread clinical use. In this work, we established a bioreactor-based manufacturing system for the production of cartilage grafts. Materials & methods: All bioprocesses, from cartilage biopsy digestion through the generation of engineered grafts, were performed in our bioreactor-based manufacturing system. All bioreactor technologies and cartilage tissue engineering bioprocesses were transferred to an independent GMP facility, where engineered grafts were manufactured for two large animal studies. Results: The results of these studies demonstrate the safety and feasibility of the bioreactor-based manufacturing approach. Moreover, grafts produced in the manufacturing system were first shown to accelerate the repair of acute osteochondral defects, compared to cell-free scaffold implants. We then demonstrated that grafts produced in the system also facilitated faster repair in a more clinically relevant chronic defect model. Our data also suggested that bioreactor-manufactured grafts may result in a more robust repair in the longer term. Conclusion: By demonstrating the safety and efficacy of bioreactor-generated grafts in two large animal models, this work represents a pivotal step towards implementing the bioreactor-based manufacturing system for the production of human cartilage grafts for clinical applications. Read the Editorial for this article on doi:10.1111/cpr.12625. | |
| Keywords | bioreactor cartilage repair large animal study bioreaktor popravilo hrustanca velika študija na živalih |