Author/Editor		Zemljič Jokhadar, Špela; Iturri, Jagoba; Toca-Herrera, Jose Luis; Derganc, Jure
Title		Cell stiffness under small and large deformations measured by optical tweezers and atomic force microscopy
Type		članek
Vol. and No.		Letnik 54, št. 12
Publication year		2021
Volume		str. 1-12
ISSN		1361-6463
Language		eng
Abstract		Cytoskeleton-disrupting drugs can have different effects on cell mechanics at different deformation scales. We therefore applied two complementary indentation techniques to study the effects of two actin-disrupting drugs on cellular stiffness of human umbilical vein endothelial cells. Optical tweezers were used to probe the cortical stiffness at small deformations, and atomic force microscopy was used to probe the bulk cell stiffness at larger deformations. The first drug studied was CK-869, which is an inhibitor of the actin branching complex Arp2/3, and has not been analysed yet in terms of mechanical effects. A significant decrease in cell stiffness upon treatment with CK-869 was measured with both techniques, which implies that actin branching is important for cell mechanics at small and large deformations. The second drug studied was jasplakinolide, for which ambiguous effects on cell mechanics have been reported. In line with previous studies, we found that jasplakinolide caused significant cell stiffening at large deformations but slight cell softening under small deformations. This result implies that jasplakinolide has different effects on different levels of actin organization.
Keywords		effects of drugs cell mechanics cytoskeleton učinki zdravil celična mehanika citoskelet