Author/Editor		Gradišnik, Lidija; Milojević, Marko; Velnar, Tomaž; Maver, Uroš
Title		Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood
Type		članek
Vol. and No.		Letnik 47, št. 9
Publication year		2020
Volume		str. 6929-6940
ISSN		1573-4978 - Molecular biology reports
Language		eng
Abstract		Macrophages are among the most important cells of the immune system. Among other functions, they take part in almost all defense actions against foreign bodies and bacteria, being particularly important in infections, wound healing, and foreign body reactions. Considering their importance for the health of the human body, as well as their important role in several diseases, the in vitro studies based on these cells, are a crucial research field. Taking all mentioned into account, this study describes a simple isolation method of human macrophages (MFUM-HMP-001 and MFUM-HMP-002 cell lines) from peripheral blood. For this purpose, the morphology, the viability, and the phagocytotic activity of the isolated cells were tested. The Immunostaining of MFUM-HMP-001 and MFUM-HMP-002 cells confirmed the macrophage cell markers CD68, CD80, and CD163/M130. The phagocytotic activity was marked in both MFUM-HMP-001 and MFUM-HMP-002 cells, as was the phagocytosis of the pHrodo green Escherichia coli bioparticles conjugates, which was enhanced with the addition of lipopolysaccharide. The cells were stable and exhibited good growth. According to our results, both cell lines are useful for the development of novel macrophage cell-based in vitro models.
Keywords		cell characterization cell isolation protocol human peripheral blood macrophages phagocytotic activity