Author/Editor | Hancock, John M.; Sumner, Adrian Thomas | |
Title | The role of proteins in the production of different types of chromosome bands | |
Type | članek | |
Vol. and No. | Letnik 35, št. 137 | |
Publication year | 1982 | |
Volume | str. 37-46 | |
ISSN | 0011-4529 - Cytobios | |
Language | eng | |
Abstract | Experiments have been carried out to try and answer two questions on the role of proteins in chromosome banding: firstly, what degree of protein extraction is required before banding can be produced; and secondly, to what extent are redistribution and reorganization of chromosomal components required for the production of banding. Partial extraction of all histones, and of a group of non-histones with molecular weights mainly between 50,000 and 70,000 appears to be necessary before G-, C- or R-banding can be produced. More extensive 'dehistonization' to produce chromosome scaffolds inhibits the production of all types of bands. Protein-protein and protein-DNA cross-linking inhibits all types of banding tested, the degree of inhibition being roughly related to the degree of cross-linking, but not apparently to the type of cross-linking. The results of both sets of experiments indicate that chromosome banding of all types is dependent on the prior loss from chromosomes of a specific set of proteins, and on some alteration of the arrangement of remaining chromosomal components during the banding procedure. | |
Keywords | proteins chromosome cross-linking proteini kromosom navzkrižno povezovanje |