Author/Editor		Avšič-Županc, T; Toney, A; Anderson, K; Chu, Y-K; Schmaljohn, C
Title		Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae
Type		članek
Source		J Gen Virol
Vol. and No.		Letnik 76
Publication year		1995
Volume		str. 2801-8
Language		eng
Abstract		We examined the genetic and antigenic properties of Dobrava (DOB) virus, a hantavirus associated with severe haemorrhagic fever with renal syndrome in Europe. Cloning and sequence analyses revealed the DOB M segment to consist of 3644 nucleotides, with a coding capacity of 1134 amino acids in the virus complementary-sense RNA (cRNA). Seven potential asparagine-linked glycosylation sites were identified in the M segment gene product, one in the G2 and six in the G1 coding regions. The S segment is 1667 nucleotides long, and has a single ORF in the cRNA capable of encoding a protein of 428 amino acids. Phylogenetic comparisons of the M and S segments of DOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly disinct from Hantaan (HTN) and Seoul (SEO) viruses. Certain G2-specific, but not G1-specific monoclonal antibodies to HTN virus reacted to the same titre with DOB and homologous viral antigen. Plaque-redaction neutralization tests indicated that, of the sera tested, only antisera to SEO virus were able to neutralize DOB virus to a titre greater than 1:10; however, this neutralization titre was eightfold lower than that observed with homologous SEO virus. The data reported here confirm that DOB virus is a unique species in the Hantavirus genus, family Bunyaviridae.
Descriptors		HANTAVIRUS RNA, VIRAL CELLS, CULTURED ENZYME-LINKED IMMUNOSORBENT ASSAY