Author/Editor | Žnidaršič, P; Pavko, A; Komel, R | |
Title | The growth form of the inducing microorganism and chitin eddition affect mycolytic enzyme production by Trichoderma harzianum | |
Type | članek | |
Source | J Ind Microbiol | |
Vol. and No. | Letnik 15 | |
Publication year | 1995 | |
Volume | str. 397-400 | |
Language | eng | |
Abstract | The effect of the growth form of the inducing microorganism on specific trichoderma harzianum mycolytic enzyme production was studied. The pelleted form of rhizopus nigricans gave a better product concerning protoplast formation ability. The maximum yield of protoplasts from the target fungus cochliobolus lunatus was 1 X 10 8ml-1. Analysis of individual specific enzyme activities in trichoderma mycolytic enzyme preparations confirms the importance of high chitinase and low protease activity for high protoplast yields. Supplementaion of the production medium with chitin increased the chitinase activity in the trichoderma exoenzyme mixture. | |
Descriptors | TRICHODERMA RHIZOPUS MYCOLOGY PROTOPLASTS CHITINASE MICROSCOPY, ELECTRON |