Author/Editor		Delorme, R; Souchier, C; Bryon, P-A
Title		A three dimensional quantitative approach for colonization study of cyclin A and P34cdk1 by confocal microscopy
Type		članek
Source		Acta Stereol
Vol. and No.		Letnik 14, št. 2
Publication year		1995
Volume		str. 155-60
Language		eng
Abstract		In mammalian cells, cyclin A and cyclin B are known to regulate P34cdkl activity during the entry into mitosis, whereas cyclin A also regulates p33cdk2 kinase. Confocal laser scanning microscopy acquisition and three dimensional image analysis were performed to quantitatively estimate cyclin A and P34cdkl colocalization on lymphoid RAMOS cell line. Cytofluorograms were use to derive parameters estimating colocalization. Colocalization globally increased when cells accumulated cyclin A. However, results demonstrated that some positive voxels were not colocalized.
Descriptors		CELLS, CULTURED CYCLINS PROTAMINE KINASE MICROSCOPY, CONFOCAL