Author/Editor		Smolar, Janja; Šturm, Blažka; Badovinac, David; Lavrin, Teja; Goričar, Katja; Dolžan, Vita; Tomažič, Aleš; Lenassi, Metka
Title		Velikost zunajceličnih veziklov je povezana s celokupnim preživetjem bolnikov z adenokarcinomom trebušne slinavke
Translated title		The size of extracellular vesicles correlates to the overall survival of patients with pancreatic adenocarcinoma
Type		članek
Publication year		2022
Volume		str. str.
ISSN		1318-0347 - Zdravniški vestnik : glasilo Slovenskega zdravniškega društva : Slovenian medical journal
Language		slv
Abstract		Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. In our study, we wanted to evaluate the correlation between extracellular vesicles (EV) from blood plasma and the overall survival rate of patients with PDAC who were treated surgically. Methods: The retrospective study included 32 subjects diagnosed with PDAC and treated surgically at the Clinical Department of Abdominal Surgery, UMC Ljubljana. For the study, we obtained clinical data, laboratory parameters, histopathologic evaluation, and blood plasma for each subject. EVs were isolated from blood plasma and characterized for their concentration and size. Results: According to the type of surgery, the subjects were divided into two groups, i.e., those with tumour resection and those with exploration without resection. We showed that EVs in the blood plasma of subjects with exploration were significantly larger than EVs of patients with resection (p < 0.05). With the help of ROC analysis, we successfully determined the EV size cut-off value, which separated subjects according to the type of surgical treatment. At a cut-off value of 183.3 nm for mean EV size, sensitivity and specificity for resection prediction are 0.591 and 0.900, respectively, with area under the curve 0.77 (95% CI = 0.60‐0.93, p = 0.016). Subjects with larger EVs had poorer overall survival (p < 0.05), whereas the survival of subjects with the average size or median size of EVs greater than the cut-off value had 13.8 months shorter overall survival (p < 0.05). Conclusion: As part of the research, we demonstrated a correlation between EV size and the overall survival of patients with PDAC who were treated surgically. We thereby demonstrated the potential of EVs as additional biomarkers for a personalized approach to treating patients with PDAC.
Keywords		pancreatic cancer exploration resection rak trebušne slinavke eksploracija resekcija