Author/Editor		Poljak, M
Title		Pomen okužbe s humanimi virusi papiloma v etiopatogenezi epitelijskih novotvorb grla in požiralnika
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1995
Volume		str. 110
Language		slo
Abstract		The human papillomaviruses (HPV) are small DNA viruses that belong to the family Papovaviridae. Infection with certain types of HPVs are intimately linked with squamous epithelial cell benign and malignant lesions of the lower genital tract in both men and women. This relationship can be appreciated on many levels, as demonstrated by a wealth of molecular, experimental, morphological, and clinical data. Despite extensive research, the role of HPV infection in the etiology of the similar epithelial lesions of the upper part of the respiratory and digestive tract remains inconclusive. In the present study 204 archival formalin fixed-paraffin embedded tissue specimens from 136 patients with benign and malignant epithelial lesions of the larynx and esophagus, were screened for HPV infection using two highly sensitive molecular methods: nonradioactive in situ hybridization (ISH) with 6-11, 16-18 and 31-33-51 HPV biotin-labelled probes and polymerase chain reaction (PCR) using two L1 consensus primer sets. For typing of HPVs after PCR, restriction fragment of the amplified products using seven restriction enzymes and enzyme linked oligonucleotide assay using typespecific probes for HPV types 6, 11, 16, 18 and 33 were performed. The quality of each DNA sample was verified initially with the amplification of portions of ubiquitous human beta-globin or beta-actin genes. Uracyl-N-glycosylase procedure was used for the prevention of the false positive results due to amplicon carry-over. Using ISH and PCR, DNA of HPV types 6-11 were detected in 28 Abstract truncated at 3200 characters.
Descriptors		LARYNGEAL NEOPLASMS PHARYNGEAL NEOPLASMS PAPILLOMAVIRUS, HUMAN IN SITU HYBRIDIZATION POLYMERASE CHAIN REACTION