Author/Editor		Geršak, K
Title		Morfometrične in funkcionalne lastnosti granuloza-luteinskih celic v pogojih naravnega in spodbujenega ciklusa
Translated title		Morphometric and functional characteristics of human granulosa-luteal cells in natural and stimulated IVF-ET cycles
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1996
Volume		str. 58
Language		slo
Abstract		Objective: to investigate human granulosa-luteal cells derived from natural and stimulated IVF-ET cycles. Setting: IVF unit, Department of Obstetrics and Gynaecology, Medicl Centre Ljubljana. Material in methods: Cells were obtained from dominant follicles of 52 women with natural cycles in which preovulatory hCG was given when the follicle was mature, and from 50 follicles of 45 women undergoing IVF-ET with hMG and hCG. Subpopulations were observed by computerized image analysis using HE and immuperoxidase staining for hCG localization. Hormone assays were done on all follicular Fluids for hCG, estradiol and progesterone. Results: Four subpopulations were larger than those from the stimulated ones. In luteinized cell types, there was no difference in cell area between natural and stimulated cycles. No differences were detected in the relative number of each of the four subpopulations within the follicular aspirates from naturally developing follicles. In stimulated ones, follicles with fertilized oocytes had more nonluteinized cells and fewer large luteinized cells. Three staining types of hCG localization were foud: on the surface membrane, on the surface membrane and within the cytoplasm, and within the cytoplasm alone. The hCG stained cells from natural cycles were larger than the ones from stimulated cycles. The natural developing follicles contained more hCG stained cells than the stimulated ones. The follicles with fertilizable oocytes had more cells with cytoplasmic hCG localization regardless type of IVF-ET protocol. Only in natural cycles there was a correlation between follicular fluid hCG levels and the percentage of the hCG stained cells. Human chorionic gonadotropin levels in natural cycles were also influenced by fertilizability of oocytes: in follicles with fertilized oocytes, follicular fluid had higher hCG levels than with nonfertilized oocytes. Conclusion: We found the differences in morphometric charaster.
Descriptors		OVULATION INDUCTION GRANULOSA CELLS FOLLICULAR FLUID LUTEIN CELLS EMBRYO TRANSFER FERTILIZATION IN VITRO GONADOTROPINS, CHORIONIC MENOTROPINS ESTRADIOL PROGESTERONE IMMUNOHISTOCHEMISTRY ADULT IMAGE PROCESSING, COMPUTER-ASSISTED