Author/Editor		Shoenfeld, Yehuda; Golburd, Boris; Hojnik, Maja; Damianovich, Maya; Hacham, Shoshana; Kopolovic, Yuri; Polak-Charcon, Iris; Afek, Arnon; Hun-Chi, Lin; Peter, James B
Title		Induction of Goodpasture antibodies to noncollagenous domain (NC1) of type IV collagen in mice by idiotypic manipulation
Type		članek
Source		Hum Antibodies Hybridomas
Vol. and No.		Letnik 6, št. 4
Publication year		1995
Volume		str. 122-8
Language		eng
Abstract		The characteristic pathogenic autoantibodies in Goodpasture's syndrome (GPS) are directed to the noncollagenous domain (NCI) of basement membrane type IV collagen. To examine whether immunization with anti-NCI antibodies could lead to GPS-like pathology, naive BALB/c mice were immunized intradermally with a mouse IgG anti-NCI monoclonal antibody or IgG serum fraction derived from patients with GPS. Mice immunized with normal mouse or human IgG and nonimmunized mice served as controls. Anti-NCI antibodies of IgG isotype were detected in the sera of mice injected with anti-NCI antibodies, but not in the sera of control mice. The presence of circulating anti-NCI antibodies coincided in some of the mice with erythrocyturia or proteinuria and pathological changes in the kidneys. No pathologic alterations were seen in the control mice. The results show that specific idiotypic manipulation can induce anti-NCI antibodies and pathological changes resembling human GPS. (Hum Antibod Hybridomas 1995; 6: 122-128)
Descriptors		AUTOIMMUNE DISEASES ANTIBODIES