Author/Editor		Jarc, M
Title		Vpliv različnih koncentracij etanola na tvorbo fosfatidiletanola in fosfatidne kisline v humanih nevroblastomskih SH-SY5Y celicah
Translated title		Effect of different ethanol concentrations on phosphatidylneuroblastoma SH-SY5Y cells
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 34, št. 3
Publication year		1995
Volume		str. 319-25
Language		slo
Abstract		The effect of ethanol on the process of signal transduction was studied in the human neuroblastoma SH-SY5Y cells. By dissolving in the cell membrane ethanol changes its chemical and physical structure. In the presence of membrane enzyme phospholipase D, a specific product phosphatidylethanol is formed, at the expense of normal formation of phosphatidic acid. Higher ethanol concentrations (25 mmol/l, 50 mmol/l, 100 mmol/l, 150 mmol/l) increasingly inhibit phosphatidiac acid formation and stimulate phosphatidylethanol synthesis. Phospatidylethanol is formed exclusively at the expense of phosphatidic acid and no other mechanism of phosphatidic acid synthesis is present. The formation of phosphatidic acid is completely inhibited by adding 150 mmol/l ethanol.
Summary		This article deals with electrophysiological characteristics of the long sensory tracts and the interneuronic system of the spinal cord in patients operated on the spinal cord. Spinal somatosensory evoked potentials (SSEP) were recorded using the subpial and the epidural technique during intraoperative neuromonitoring, which is obligatory in all operations on the spinal cord performed at the Department of Neurosurgery, Ljubljana. The median, tibial and sural nerves were stimulated. Sopmatosensory evoked potentials of the long sensory tracts and of the spinal interneuronic system consist of different elements. Changes in these elements indicate domage to the parts of the spinal cord that generate them. The most stable element of the conducted SSEP are the intial negative waves, which originate in the spinocerebellar tract. Deterioration in the shape of the negative high amplitude potentials (NHP) is the most sensitive indicator of damage to the somatosensory system of the spinal cord. Their disappearance or absence indicate severe damage to the dorsal columns. The negative low amplitude potentials (NLP) are the most sensitive element of the conducted SSEP. Their absence indicates damage to the samotosensory system of the spinal cord. Wich can not be precisely localized. The N11 and N14 waves are the most stable elements of the cevical and lumbo-sacral interneuronic SSEP. Since they remain unchanged even in cases of severe domage to the dorsal columns they most probably originate in the dorsal root entry zone. The N13 and N17 waves are the most frequently changed elements of the cervical and lumbo-sacral interneuronic SSEP. Changes of these two waves suggest the damage to the spinal cord gray matter. High frequency waves seen on the slow negative wave of the interneuronic SSEP or appearing in the place of the absent N13 wave most probably reflect the activity of the somatosensory long tracts.(krajš.)
Descriptors		NEUROBLASTOMA ALCOHOL, ETHYL PHOSPHATIDIC ACIDS TUMOR CELLS, CULTURED