Author/Editor		Bundgaard, MJ; Regeur, L; Troncoso, J; West, MJ; Pakkenberg, B
Title		Cell volume of neocortical neurons from patients with Alzheimer's disease
Type		članek
Source		Acta Stereol
Vol. and No.		Letnik 15, št. 2
Publication year		1996
Volume		str. 119-24
Language		eng
Abstract		The unbiased estimation of total neuron number in neocortices of five patients, age range 79 to 88 yrs with Alzheimer's disease was compared with total neocortical neuron number in four non-demented controls, age range 70 to 101 yrs. The demented patients were from a longitudinal study on Alzheimer's disease from Johns Hopkins University Hospital (JHUH) in Baltimore, USA. The total nerve cell number in the Alzheimer group was 19.7.10 9 with a coefficient of variation (CV) = 0.17, while total neuron number in the control group was 18.6.10 9, CV = 0.08, the difference of 6 percent is obviously not significant. Each sampled neocortical neuron was measured in a semiautomatic procedure using the menu-driven computer program CAST-GRID (Olympus, Denmark). The rotator, the unbiased stereological principle by which an estimate of the volume of an object can be obtained by rotating a 3-D object around an axis in the plane, was used to obtain a unbiased estimate of the mean cell volume and size distribution of neocortical neurons. We found a statistically significant incrase in the size of the neuronal nuclei in neocortices from patients with Alzheimer's disease from JHUH: 351 micro m3 (CV = = 0.07) vs. 287% increase in neuron perikaryon volume from 1146 micro m3 (CV = 0.11) in controls to 1197 micro m3 (CV = 0.13) in the Alzheimer cases was not statistically significant.
Descriptors		ALZHEIMER'S DISEASE CEREBRAL CORTEX NEURONS AGED HISTOLOGICAL TECHNIQUES