Author/Editor | Knežević, M; Alscher, M; Žunec, P; Hartman-Pretnar, K; Rožman, P; Čurin-Šerbec, V | |
Title | Cultivation of mouse-mouse hybridomas producing IgM monoclonal antibodies | |
Type | članek | |
Source | Pflugers Arch | |
Vol. and No. | Letnik 431, št. 6 Suppl 2 | |
Publication year | 1996 | |
Volume | str. R231-2 | |
Language | eng | |
Abstract | The mouse-mouse hybridomas producing monoclonal antibodies (MAbs) for ABO blood group determination were cultivated for 10 days in 25 cm/2 Roux bottles using standard cultivation medium (DMEM) with different foetal-calf serum (FCS) concentrations (2-13%). The highest specific production rates (200-1100 micro g/10/6 cells/day) for MAbs were measured at the end of the cultivation: this phenomenon could be explained by advanced cell death and liberating the content of the cells into the medium. | |
Descriptors | ANTIBODIES, MONOCLONAL ABO BLOOD-GROUP SYSTEM HYBRIDOMAS MICE CELL LINE IGM |