Author/Editor		Juntes, P
Title		Improvement of the method for demonstration of nucleolar organizer regions (NORs) in formalin fixed and paraffin embedded tissue using microwave pretreatment
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 431, št. 6 Suppl 2
Publication year		1996
Volume		str. R269-70
Language		eng
Abstract		For demonstration, accurate evaluation and measurements of nucleolar organizer regions (NORs) in nuclei, a good quality reaction of silver staining is required. On formalin fixed and parafin embedded tissue this can be achieved only, if special procedures are used. We managed to get sharply delineated, dark brown or black dots representing AgNORs (silver stained NORs), that are easy to count and measure, with microwave pretreatment of tissue sections before incubation in silver solution.
Descriptors		MAMMARY NEOPLASMS SILVER STAINING NUCLEOLUS ORGANIZER REGION MICROWAVES ANIMALS, DOMESTIC DOGS