Author/Editor     Horvat-Žnidaršič, I; Šuput, D
Title     The effect of equinatoxin II on nerve and muscle
Type     članek
Source     Pflugers Arch
Vol. and No.     Letnik 431, št. 6 Suppl 2
Publication year     1996
Volume     str. R279-80
Language     eng
Abstract     The effect of equinatoxin II on the excitability of nerve and muscle membrane was examined. Neuromuscular transmission was assessed indirectly by measurement of isometric muscle contractions. Membrane currents and potentials were measured by means of vaseline-gap voltage-clamp and current-clamp methods on single isolated skeletal muscle fibres. 10 nM equinatoxin II induces spontaneous twitches of skeletal muscle fibres provided that the neuromuscular junction remains intact. In curarized muscles and by use of higher final concentrations of equinatoxin II prolonged contractures were observed. This may be explained by a pore formation in the muscle membrane. An increase in the leakage conductance of skeletal muscle fibres supports this hypothesis.
Descriptors     MUSCLE CONTRACTION
SYNAPTIC TRANSMISSION
CNIDARIAN VENOMS
RANIDAE
PATCH-CLAMP TECHNIQUES