Author/Editor		Šentjurc, M; Štalc, A; Šuput, D
Title		Influence of eqinatoxin II on coronary smooth muscle membrane fluidity
Type		članek
Source		Pflugers Arch
Vol. and No.		Letnik 431, št. 6 Suppl 2
Publication year		1996
Volume		str. R317-8
Language		eng
Abstract		Equinatoxin II forms pores in artificial as well as in natural phospholipid bylayers. As it also reduces the perfusion through the coronary arteries it seemed reasonable to investigate the interaction of this protein with the plasmalemma of the vascular smooth muscle cells more directly with the patch-clamp method and with EPR using the spin probe methyl ester of 5-doxylpalmitate. In the EPR spectra at least three regions in the membranes were identified with different membrane fluidity. Application of 0.1 microM or 1 microM equinatoxin II increases the portion with the lower membrane fluidity, and irreversibly increases the unspecific membrane conductance. This is compatible with the view that equinatoxin II forms pores in the membrane bilayer.
Descriptors		MUSCLE, SMOOTH, VASCULAR CNIDARIAN VENOMS MEMBRANE FLUIDITY CORONARY VESSELS PATCH-CLAMP TECHNIQUES RATS ANIMALS, LABORATORY