Author/Editor		Poljak, M; Gubina, M; Žabkar, SM; Tepeš, B; Kavčič, B
Title		Verižna reakcija s polimerazo v diagnostiki okužb s Helicobacter pylori
Translated title		Polymerase chain reaction in doagnosis of Helicobacter pylori infections
Type		članek
Source		In: Cerar A, Križman I, editors. Gastritis in z njim povezane bolezni. 27. memorialni sestanek profesorja Janeza Plečnika. Ljubljana: Medicinska fakulteta, Inštitut za patologijo,
Publication year		1996
Volume		str. 54-7
Language		slo
Abstract		Backgraund. The aim of the present study was to evaluate the usefulness of two polymerase chain reaction (PCR) methods - one for the detection of Helicobacter pylori in biopsy specimens and another for molecular-epidemiology studies of slovenian H. pylori strains. Results and conclusions. 16SrRNA-based PCR assay for the detection of H. pylori was compared with three standard diagnostic methods on 26 fresh gastric biopsy specimens. Bacteria were found in 23, 19, 24, and 26 samples, by PCR, culture, the rapid-urease test, and histology, respectively. Archival formalin-fixed biopsy specimens were found inappropriate for the molecular analyses. ureB PCR coupled by restriction fragment length polymorphism of PCR products was found to be suitable for analyzing of the genetic heterogenity of H. pylori isolates obtained from patients in Slovenia.
Descriptors		HELICOBACTER INFECTIONS HELICOBACTER PYLORI POLYMERASE CHAIN REACTION