Author/Editor		Geršak, K; Tomaževič, T
Title		Subpopulations of human granulosa-luteal cells in natural and stimulated IVF-ET cycles
Type		članek
Source		In: Aburumieh A, Bernat E, Dohr G et al, editors. 9th world congress on in vitro fertilization and asssisted reproduction; 1995 Apr 3-7; Vienna. Bologna: Monduzzi editore,
Publication year		1995
Volume		str. 191-4
Language		eng
Abstract		The aim of the study was to find out the differences between human granulosa and granulosa-luteal cells derived from 15 natural and 40 stimulated IVF-ET cycles. Four subpopulations of cells were observed by computerized image analysis: nonluteinized cells, small, medium size and large luteinized cells. Human chorionic gonadotropin (hCG) was localized using immunoperoxidase staining: on the surface membrane, on the surface membrane and within the cytoplasm, and only within the cytoplasm. The nonluteinized granulosa cells and all types of hCG stained cells from natural cycles were larger than those from the stimulated ones. The natural developing follicles contained also the higher number of hCG stained cells than the stimulated ones. The follicles with fertilizable oocytes had more cells with cytoplasmic hCG localization. Only in natural cycles there was a negative correlation between follicular fluid (FF) hCG levels and the percentage of cells with membrane hCG localization, and a positive correlation between FF hCG levels and the percentage of cells with cytoplasmic hCG localization. We found the differences in morphometric characteristics and hCG localization between human granulosa and granulosa-luteal cells obtained from natural and stimulated IVF-ET cycles.
Descriptors		FERTILIZATION IN VITRO EMBRYO TRANSFER GRANULOSA CELLS LUTEIN CELLS CORPUS LUTEUM ESTRADIOL GONADOTROPINS, CHORIONIC OVULATION INDUCTION MENOTROPINS OOCYTES