Author/Editor		Ihan, A
Title		Analiza imunskih celic s pretočnim citometrom
Translated title		Flow cytometry for immune cell analysis
Type		članek
Source		Farm Vestn
Vol. and No.		Letnik 47, št. 3
Publication year		1996
Volume		str. 275-9
Language		slo
Abstract		Flow cytometry has been introduced as the most reliable method for the analysis of lymphocytes ubsets. The advantage of it is that it enables a quantitative analysis of large cell populations, and cells can be simultaneously analysed for multiple marker expresion. The latter enables the analysis of different functional molecules (cytokine receptors, adhesion molecules, activation markers) on distinct lymphocyte subpopulations. This may provide new information about immunopathogenetic mechanisms in inflamed tissues. In the article we described our experiences in flow cytometry analysis of lymphocytes infiltrating distinct inflamed tissues (nasal polyps, gastric mucosa, cerebrospinal fluid).
Summary		Pretočna citometrija se je uveljaviila kor primerna metoda za analizo limfocitnih populacij. Omogoča kvantitaativno analizo obsežnih celičnih populacij, pri tem pa je mogoče vsako celico posebej analizirati glede izražanja več molekul hkrati. To omogoča opazovanje različnih funkcionalnih molekul (receptorjev za citokine, adhezijskih molekul, aktivacijskih markerjev) na izbranih vrstah celic. Taki eksperimenti omogočajo mnoga nova spoznanja o imunskem dogajanju v vnetih tkivih. V članku opisujemo nekaj naših izkušenj z analizo imunskih celic v posameznih vnetih tkivih (nosnih polipih, vneti želodčni sluznici bolnikov s peptično razjedo, cerebrospinalni tekočini).
Descriptors		ANTIGENS, CD FLOW CYTOMETRY LYMPHOCYTES IMMUNITY, NATURAL MICROSCOPY, FLUORESCENCE