Author/Editor		Poljak, M; Seme, K
Title		Rapid detection and typing of human papillomaviruses by consensus polymerase chain reaction and enzyme-linked immunosorbent assay
Type		članek
Source		J Virol Methods
Vol. and No.		Letnik 56
Publication year		1996
Volume		str. 231-8
Language		eng
Abstract		A novel method for the detection and typing of human papillomaviruses (HPV) based on consensus polymerase chain reaction (PCR) using MY09/MY11 primers followed by detection of PCR products in a standard microtiter plate format using a recently developed commercially available standardised PCR ELISA kit (Boehringer Mannheim, Germany) was developed. The reliability and feasibility of the method were evaluated on 140 HPV-positive and 85 HPV-negative DNA samples extracted from different archival clinical specimens.Virtually complete agreement between the results of this novel method and the results of previous in-house PCRs and typing method was obtained. The sensitivity level of the novel method, determined by serial log-dilutions of SiHA cells, is about 50 copies of HPV 16. The PCR-ELISA provides the potential for an automated, simple, rapid and accurate test for detection and typing of HPV in diagnostic virological laboratories.
Descriptors		PAPOVAVIRIDAE INFECTIONS DNA, VIRAL POLYMERASE CHAIN REACTION TUMOR VIRUS INFECTIONS CONSENSUS SEQUENCE PAPILLOMAVIRUS, HUMAN TUMOR CELLS, CULTURED SENSITIVITY AND SPECIFICITY FEASIBILITY STUDIES BASE SEQUENCE MOLECULAR SEQUENCE DATA NUCLEIC ACID HYBRIDIZATION