Author/Editor		Pokrajac, T; Wraber, B; Gubina, M; Dragaš, AZ; Koren, S
Title		Measurement of concentration of inflammatory cytokines and soluble interleukin-2 receptor in human blood
Type		članek
Source		In: Faist E, editor. The immune consequences of trauma, shock and sepsis: mechanisms and therapeutic approaches. 4th international congress; 1997 Mar 4-8; Munich. Bologna: Monduzzi editore,
Publication year		1997
Volume		str. 7-11
Language		eng
Abstract		Soluble mediators secreted by the cells of immune system are involved in the pathophysiology of stress, inflammation, sepsis, autoimmune diseases and malignant diseases. Some of the most important soluble mediators are tumour necrosis factor (TNF), interleukin-1 (IL-1) and interleukin-6 (IL-6), which are classified as cytokines. Soluble interleukin-2 receptor (s-IL-2RR) is a soluble cytokine receptor found to be in direct proportion to the membrane IL-2 receptors on CD3+ lymhocytes, whiche are, together with IL-2 implicated to the specific immune response. The puropse of this research work was to determine the concentrations of cytokines (TNF, IL-1beta, IL-6) and s-IL-2R in the serum and plasma of healthy blood donors. We measured the concentrations of cytokines and soluble receptors in: freshly prepared plasma, plasma prepared 24 hours after the blood was taken and in the serum. We then compared the concentrations of cytokines and s-IL-2R in the plasma and serum using different reagents. Blood was taken from 29 healthy donors to determine cytokine concentration and from 64 healthy donors to determine s-IL-2R concentration. The concentrations fo cytokines and s-IL-2R were measured using commercial RIA (Amersham, Great Britain) and ELISA (Endogen, USA; Eurogenetics, Belgium: Innotest hTNFalpha, Belgium; T-Cell Sciences, USA) tests. We determined a normal range of concentrations for each mediator. The mean values for the concentrations of cytokines and receptors in healthy blood donors were the same for both fresh plasma and plasma prepared after 24 hours. The mean values for the concentrations of cytokines and receptors in fresh plasma and in serum also did not differ from one another. However, the TNF concentration in serum was significantly higher than the concentration in fresh plasma when the innotest hTNFalpha tests were used. The RIA and ELISA tests give different results and thus are not directly comparable with one (Abstract truncated at 2000 characters).
Descriptors		CYTOKINES RECEPTORS, INTERLEUKIN-2 ADULT MIDDLE AGE