Author/Editor		Coer, Andrej; Gale, Nina; Kambič, Vinko
Title		Quantitative pathology of laryngeal epithelial hyperplastic lesions
Type		članek
Source		Acta Otolaryngol Suppl (Stockh)
Vol. and No.		Letnik 527, št. Suppl
Publication year		1997
Volume		str. 57-61
Language		eng
Abstract		We studied 40 laryngeal biopsies samoples in order to ascertain the reliability of light microscopical criteria for subdividing laryngeal epithelial hyperplastic lesions (EHL) and carcinoma in situ as well as to determine the relationship between proliferative activity of their epithelial cells and the histological grade. The biopsies were divided into four groups in accordance with the Kambi;-Lenart classification: simple, abnormal and atypical hyperplasia and carcinoma in situ. 10 cases in each group were included. The morphometrical analysis was carried out by a semiautomatic image analysis system. The proliferative activity was determined by the high percentage of cell nuclear antigen (PCNA) and Ki-67 positive epithelial cells and with counting nucleolar organizer regions (Ag-NORs) per nucleus. Our results suggest that measuring the nuclear area of the basal cells, augmented with basaloid cells and carcinomatous cells, is the most useful morphometrical method of differentiating three types of laryngeal EHL and carcinoma in situ, while the proliferative activity progressively increased with the degree of epithelial hyperplasia. Morphometrical methods and proliferative activity should be regarded as useful in conjuction with the traditional histopathological methods allowing more of objective grading of EHL.
Descriptors		LARYNGEAL NEOPLASMS HYPERPLASIA EPITHELIUM PRECANCEROUS CONDITIONS CARCINOMA IN SITU