Author/Editor		Ferluga, Dušan; Luzar, Boštjan; Vodovnik, Aleksandar; Poljak, Mario; Coer, Andrej; Gale, Nina; Kambič, Vinko
Title		Langerhans cells in human papillomaviruses types 6/11 associated laryngeal papillomas
Type		članek
Source		Acta Otolaryngol Suppl (Stockh)
Vol. and No.		, št. Suppl 527
Publication year		1997
Volume		str. 87-91
Language		eng
Abstract		Some studies have shown a reduced density of Langerhans cells (LCs) within epithelium infected by human papillomaviruses (HPV) types 16/18. However, data on a correlation between HPV types 6/11 infection and LCs have been missing. To solve this problem, we analysed 24 biopsy specimens of laryngeal papillomas, selected randomly, 20 multiple and 4 solitary. The presence of HPV 6 and 11 was proven by polymerase chain reaction (PCR) using 2 different sets of primers in 23 biopsy specimens. Abnormalities of the covering stratified squamous epithelium were graded according to the Kambič-Gale classification.. LCs were immunohistochemically labelled with 2 different antibodies, CD1a and S100. Quantitative analysis was performed to determine the density of LC per mm/2 in different grades of epithelial abnormalities covering laryngeal papillomas. Although no statistically significant differences in the mean number of LCs per mm/2 of the cross-sectioned epithelium covering laryngeal papillomas were observed comparing simple, abnormal and atypical hyperplasia groups, the mean number of LCs per mm/2 in laryngeal papillomas associated with HPV types 6/11 infection substantially exceeded that of the vocal cord surface epithelium in patients with chronic laryngitis.
Descriptors		PAPILLOMA LARYNGEAL NEOPLASMS LANGERHANS CELLS PAPILLOMAVIRUS, HUMAN BIOPSY POLYMERASE CHAIN REACTION