Author/Editor		Kovačič, B; Vlaisavljević, V
Title		Slow freezing of mouse embryos using a portable computer-controlled freezing device
Type		članek
Source		Hum Reprod Update
Vol. and No.		Letnik 1, št. 3
Publication year		1995
Volume		str. 1-3
Language		eng
Abstract		The aim of this study was to establish the applicability of a portable computer-controlled freezing device, freeze control L863. Using a slow freezing technique, 290 4- and 8-cell mouse embryos were frozen in 1.5 M 1,2-propanediol. The success of the cryopreservation procedure was expressed as the percentage of thawed embryos reaching the blastocyst stage in vitro. Cryopreservation was successful for 76.9% (103 out of 134) of 4-cell embryos and 72.4% (113 out of 156) of 8-cell embryos (P<0.001). The successful slow freezing of mouse embryos with this device proves that this simple freezing device is as effective as the larger and more popular models.
Descriptors		EMBRYO CRYOPRESERVATION MICE FREEZING COMPUTERS