Author/Editor		Schweiger, Ana; Štabuc, Borut; Popović, Tatjana; Turk, Vito; Kos, Janko
Title		Enzyme-linked immunosorbent assay for the detection of total cathepsin H in human tissue cytosols and sera
Type		članek
Source		J Immunol Methods
Vol. and No.		Letnik 201
Publication year		1997
Volume		str. 165-72
Language		eng
Abstract		An enzyme-linked immunosorbent assay (ELISA) was constructed for the determination of total human cathepsin H concentration in clinical samples. Utilising monoclonal and polyclonal antibodies, raised to human liver cathepsin H. the assay is able to detect a mature protein, a precursor molecule and enzyme-inhibitor complexes. The test system permits sensitive and reliable detection of analyte either in tissue cytosols or in sera. The detection limit is 2 ng/ml (n = 10, mean of zero standard +- 3 SD). The average recovery of cathepsin H, added to the low content samples, was 95.3% +- 18%. The within-run and between-run coefficient of variance (CV) varied from 23% to 8.9% and 12.7% to 16.4%, respectively, indicating satisfactory reproducibility of the method. The level of cathepsin H was defined in tissue cytosols of human heart, muscle and kidney and in sera from 30 healthy individuals. Additionally, cathepsin H was measured in sera from 55 patients with primary skin melanoma and from 42 patients with metastatic melanoma. The mean cathepsin H level was significantly higher for both groups of patients compared to normal sera level, being highest for metastatic melanoma patients.
Descriptors		MELANOMA CATHEPSINS ENZYME-LINKED IMMUNOSORBENT ASSAY NEOPLASM METASTASIS CYTOSOL HEART KIDNEY ANTIBODIES MUSCLES