Author/Editor		Walter, I
Title		Distribution of S-100ao (alpha alpha) and S-100b (beta beta) protein in the bovine oviduct epithelium during the estrus cycle
Type		članek
Source		In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta,
Publication year		1997
Volume		str. 35-8
Language		eng
Abstract		The Fallopian tube is of considerably importance for reproduction and fertility. It functions mechanically to transport gametes and establishes the optimum environment for endmaturation of oocytes, for capacitation of spermatozoa, and for fertilization and early embryonic development. S-100 is a cytoplasmic acidic, soluble, calcium binding protein that is composed of alpha and beta subunits. Subunits contribute to three different dimers: S-l00ao (alpha beta), S- l00a (alpha alpha) and S-l00b (beta beta). S-100 protein was primariliy assumed specific for nervous t!ssue but recently has been identified, in a series of mammalian cells and tissues, such as oviduct epithelium. The distribution of S-l00ao and b in different segments of the bovine oviduct at various stages of the estrus cycle was studied by means of immunohistochemistry. In fimbriae and ampullae of the oviduct, S-l00ao and S l00b immunoreactivity was diffusely located in the cytoplasm of ciliated and non-ciliated cells. No changes were noted during the estrus cycle. Apical cytoplasmic protrusions of non-ciliated cells, which were most prominent during luteal phase, markedly stained with S-100b. In the isthmus, secretory cells showed no immunoreaction for S-IOOb but stained for S-l00ao. This staining behaviour clearly differed from that of non-cilicted cells in the ampullary and fimbrial segments, where non-ciliated cells reacted for both S-l00a,o and S-l00b. Immmunoreactivity S-100 protein subtypes in the oviduct isthmus did not change during estrus cycle. It is assumed that different staining patterns for S-100 proteins reflect different cellular functions in the oviduct segments, in particular of secretory cells.
Descriptors		FALLOPIAN TUBES ESTRUS PROTEIN S CATTLE