Author/Editor | Strmšnik, L; Števanec, A; Bavdek, SV; Mrzel, I | |
Title | Histological, immunohistochemical and quantitative analysis of the islets of Langerhans in the ostrich (Struthio camelus) | |
Type | članek | |
Source | In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta, | |
Publication year | 1997 | |
Volume | str. 129-32 | |
Language | eng | |
Abstract | Histological, immunohistochemical and quantitative characteristics of the endocrine parts of the pancreas were researched in ten ostriches (Struthio camelus) of different ages, from three weeks to ten and a half months. The histological slices were stained with haematoxylin-eosin, toluidine blue, after Goldner and with periodic acid - Schiff reaction. An immunohistochemical reaction using primary antibodies against glucagon, insulin and somatostatin, and the method employing biotin-avidin was used to show cells A, B and D. The splenic lobe of the pancreas has the largest proportion of the islets of Langerhans (26.05%), the ventral lobe has 2.54% and the dorsal lobe 1.02%. Glucagon producing A cells are presented only in the splenic (8.07%) and ventral (0.64%) lobes, insulin producing B cells and somatostatin producing D cells are also in the dorsal lobe. The proportion of B cells in the splenic lobe (7.84%), dorsal lobe (0.50%) and ventral lobe (1.28%), and the proportion of D cells in the splenic lobe (5.08%), dorsal lobe (0.28%) and ventral lobe (0.51%) was determined. The greatest part of the whole pancreatic tissue is occupied by insulin producing cells, 1.35%; 0.78% by glucagon producing cells and 0.64% by somatostatin producing cells. Individual D cells were located in the acinar exocrine pancreas. | |
Descriptors | ISLETS OF LANGERHANS IMMUNOHISTOCHEMISTRY |