Author/Editor     Strmšnik, L; Števanec, A; Bavdek, SV; Mrzel, I
Title     Histological, immunohistochemical and quantitative analysis of the islets of Langerhans in the ostrich (Struthio camelus)
Type     članek
Source     In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta,
Publication year     1997
Volume     str. 129-32
Language     eng
Abstract     Histological, immunohistochemical and quantitative characteristics of the endocrine parts of the pancreas were researched in ten ostriches (Struthio camelus) of different ages, from three weeks to ten and a half months. The histological slices were stained with haematoxylin-eosin, toluidine blue, after Goldner and with periodic acid - Schiff reaction. An immunohistochemical reaction using primary antibodies against glucagon, insulin and somatostatin, and the method employing biotin-avidin was used to show cells A, B and D. The splenic lobe of the pancreas has the largest proportion of the islets of Langerhans (26.05%), the ventral lobe has 2.54% and the dorsal lobe 1.02%. Glucagon producing A cells are presented only in the splenic (8.07%) and ventral (0.64%) lobes, insulin producing B cells and somatostatin producing D cells are also in the dorsal lobe. The proportion of B cells in the splenic lobe (7.84%), dorsal lobe (0.50%) and ventral lobe (1.28%), and the proportion of D cells in the splenic lobe (5.08%), dorsal lobe (0.28%) and ventral lobe (0.51%) was determined. The greatest part of the whole pancreatic tissue is occupied by insulin producing cells, 1.35%; 0.78% by glucagon producing cells and 0.64% by somatostatin producing cells. Individual D cells were located in the acinar exocrine pancreas.
Descriptors     ISLETS OF LANGERHANS
IMMUNOHISTOCHEMISTRY