Author/Editor     Juntes, P; Drobnič-Košorok, M; Pogačnik, M
Title     Applicability of antihuman cathepsins on canine tissues
Type     članek
Source     In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta,
Publication year     1997
Volume     str. 147-50
Language     eng
Abstract     Cathepsins are phylogeneticaly old proteins and can be found in lower and higher animal species. Since they have been related to the process of metastases formation. The interest for cathepsins as well as for their inhibitors is growing lately. It seems that their amount is in a correlation to the malignancy potential of tumors. The aim of this work was to test and estimate the suitability of antihuman cathepsins for application on animal tissues. The specifc emphasis was put on canine tissues, because of the potential use in tumor studies in this species. We tested polyclonal antihuman cathepsins B, C, D and L. Reactions were made on paraffin tissue sections of canine liver. Ovine, monkey and human liver were used for control. All tissues were routinely fixed in l0% buffered formalin. ln immunohistochemical procedure streptavidin biotin complex was applied. One series of tissue sections was pretreated with microwaves before the incubation with primary antibodies. Antihuman antibodies were only partially suitable for the use on dog tissue. Nonlyaphilized antibodies against cathepsin B reacted in human and monkey liver in a shape of small intracytoplasmic granules, which were probably lyzosomes; with anticathepsin C we got weak diffuse reaction in the cytoplasm of some hepatocytes in human and ovine liver, while in the liver of a dog the reaction was considered unspecific. Very similar reaction occured in the liver of a man and a monkey with anticathepsin D. Reaction against cathepsin L was negative in all cases. After the pretreatment of tissue sections with microwaves it was clear that the antigens were demasked on both, canine and human tissue. The same effect was reached after applying lyophilized or nonlyophilized antibodies. The reaction in dog liver was slightly weaker than in human tissue.
Descriptors     CATHEPSINS
NEOPLASM METASTASIS
DOGS
IMMUNOHISTOCHEMISTRY