Author/Editor | Cimerman, Nina; Drobnič-Košorok, Marinka; Korant, Bruce D; Turk, Boris; Turk, Vito | |
Title | Characterization of cystatin C from bovine parotid glands: cysteine proteinase inhibition and antiviral properties | |
Type | članek | |
Source | Biol Chem Hoppe Seyler | |
Vol. and No. | Letnik 377, št. 1 | |
Publication year | 1996 | |
Volume | str. 19-23 | |
Language | eng | |
Abstract | Cystatin C, a low Mr cysteine proteinase inhibitor was isolated from bovine parotid glands by a procedure which includes alkaline treatment of the homogenate, affinity chromatography, gel filtration and ion exchange chromatography. The purified inhibitor has a pl of 8.0 and Mr of 14500. The identity with bovine cystatin C from colostrum was confirmed by N-terminal sequence of the inhibitor and amino acid composition. Cystatin C rapidly (kass = 5.5 x 10(7) M-1s-1) and tightly inhibits papain (Ki = 0.02 nM), whereas its interaction with bovine cathepsin B is substantially weaker (Ki = 4.4 nM). Bovine cystatin C also shows a weak antiviral effect on poliovirus infected human Hela cells. | |
Descriptors | ANTIVIRAL AGENTS CYSTATINS CYSTEINE PROTEINASE INHIBITORS PAROTID GLAND AMINO ACID SEQUENCE CATTLE CYTOPATHOGENIC EFFECT, VIRAL KINETICS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT PLAQUE ASSAY POLIOVIRUSES |