Author/Editor		Glavač, Damjan; Dean, Michael
Title		Applications of heteroduplex analysis for mutation detection in disease genes
Type		članek
Source		Hum Mutat
Vol. and No.		Letnik 6, št. 4
Publication year		1995
Volume		str. 281-7
Language		eng
Abstract		Double-stranded heteroduplex molecules that form between a mutant and wild-type DNA strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the HA method have been developed that increase the sensitivity of detection of single-base pair alterations.
Descriptors		DNA MUTATIONAL ANALYSIS GENETIC SCREENING MUTATION NUCLEIC ACID HETERODUPLEXES CYSTIC FIBROSIS CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR GENES, VIRAL HISTOCOMPATIBILITY TESTING HIV ANTIGENS POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL SENSITIVITY AND SPECIFICITY