Author/Editor		Zajc, Irena
Title		Repetitive DNA sequences in different breeds of domestic dogs
Translated title		Ponavljajoča se zaporedja DNA pri različnih pasmah domačega psa, Canis familiaris
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1996
Volume		str. 162
Language		eng
Abstract		Microsatellite and minisatellite sequences are two classes of polymorphic DNA that are commonly found in mammalian DNA. With their extensive variability, stemming from the differences in the number of repeats of a basic sequence, both classes of repetitive DNA have proven to be valuable genetic markers. For this research, the genetic variability of 19 canine microsatellite loci was examined within and among three pure breeds of dogs; Irish Greyhounds, Labradors and German Shepherds. DNA needed for the analysis was mainly extracted from minute samples of blood. Some alternative sources of DNA, like dog hair and toenails were also examined. Microsatellite sequences were amplified separately or in multiplex PCR, using canine specific oligonucleotide primers, resolved on native vertical polyacrylamide gels, stained in ethidium bromide and visualised under UV light. From allele frequencies, the statistics that express polymorphism within a breed and exclusion probabilities were calculated and the canine breeds were compared to each other. All canine microsatellites analysed in this research were polymorphic in a limited population of unrelated dogs belonging to 15 different breeds. A considerable reduction of polymorphism was observed in the three pure breeds tested. Although at some loci breed specific alleles were observed, the significant differences among the three breeds are not in the presence or absence of certain alleles or their size range at individual locus, but in the relative frequency and distribution of these alleles across a locus. Labradors are the most polymorphic and Greyhounds the least polymorphic of the three examined breeds. The differences were higher between Labradors and Greyhounds than between Labradors and German Shepherds or German Shepherds and Greyhounds. A practical application of population data collected on microsatellites is shown with the examples of paternity and parentage testing. (Abstract truncated at 2000).
Descriptors		DOGS SEQUENCE ANALYSIS, DNA DNA, SATELLITE MICROSATELLITE REPEATS ANIMALS, DOMESTIC POLYMORPHISM (GENETICS) POLYMERASE CHAIN REACTION OLIGONUCLEOTIDE PROBES ALLELES PATERNITY DNA FINGERPRINTING