Author/Editor		Kloboves-Prevodnik, Veronika
Title		EGFr and c-erb B2 encogene overexpression in diploid and aneuploid non-malignant and malignant thyroid lesions
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1997
Volume		str. 117
Language		slo
Abstract		Aneuploid DNA content is found not only in malignant, but also in some benign thyroid lesions. Aneuploidy is most probably a consequence of chromosomal abeirations and is sometimes associated with an increased mutation rate. Epidermal growth factor receptor (EGFr) and c-erb B2 over-expression is usually associated with gene amplification and/or gene rearrangement. We expected that the over-expression of these genes is more common in aneuploid than in diploid benign and malignant thyroid lesions. AIM. To analyse EGFr and c-erb B2 gene expression in connection with the DNA ploidy values of non-neoplastic, benign and malignant neoplastic lesions. MATERIALS and METHODS. 97 thyroid lesions (29 multi-nodular goiters, 33 adenomas, 11 follicular and 26 papillary carcinomas) and 32 normal thyroids have been included in our study. Gene expression was studied at the mRNA and protein levels. RNA was extracted from formalin-fixed and paraffin-embedded tissue. Reverse transcription, PCR and dot blot were used for mRNA quantitation. The streptavidin biotin complex immunohistochemical method was used for the gene expression study at protein level. Flow cytometric DNA analysis was performed on a Partec PAS II flow cytometer. RESULT. Between the diploid and aneuploid benign and malignant thyroid lesions, EGFr and c-erb B2 mRNA levels were not significantly difFerent (p=NS). EGFr mRNA levels were higher than the basal expression in 20% of goiters, 42% of adenomas, 0% of follicular, and 19% of papillary carcinomas. c-erb B2 mRNA levels were higher than the basal expression in ?7% goiters, 4?% of adenomas 9% follicular and 15 % papillary carcinomas. Only in adenomas were EGFr and c-erb B2 mRNA levels significantly higher than the basal expression (p=0,05). In a few goiters and adenomas an increased co-expression of both genes was observed. (Abstract truncated at 2000 character)
Descriptors		THYROID DISEASES ANEUPLOIDY DIPLOIDY RECEPTORS, EPIDERMAL GROWTH FACTOR-UROGASTRONE ONCOGENE PROTEINS V-ERBB GENES, ERBB-2 RNA, MESSENGER FLOW CYTOMETRY POLYMERASE CHAIN REACTION GOITER ADENOMA CARCINOMA, PAPILLARY, FOLLICULAR THYROID NEOPLASMS DNA